What do you need?
- A ripe banana
- Measuring cup
- Water
- Table salt
- Dishwashing liquid or shampoo
- A coffee filter or cheesecloth
- A plastic container
- A wooden stick or spoon
- Isopropyl alcohol (optional)
Step 1: Prepare the Banana Mixture
Start by peeling the ripe banana and mashing it in a plastic container until it becomes a smooth paste. Add ½ teaspoon of salt and ½ cup of water to the mixture, then stir it gently with a wooden stick or spoon until the salt dissolves completely.
Step 2: Break Down the Banana Cells
Add around 2 teaspoons of dishwashing liquid or shampoo to the mixture and stir it slowly. The soap or detergent breaks down the cell membranes and releases the DNA from the banana cells.
Step 3: Filter the Mixture
Place a piece of coffee filter or cheesecloth over another container or glass to create a filter. Carefully pour the banana mixture through the filter, allowing the liquid to pass through while collecting the solid residue.
Step 4: Collect the DNA
Using a wooden stick or spoon, gently press the solid residue collected on the filter to extract as much liquid as possible. This liquid contains the banana DNA!
Step 5: Optional Alcohol Precipitation (Advanced)
If you want to visualize the extracted DNA, you can perform an additional step. Take the liquid containing the DNA and slowly pour it into a test tube or a narrow glass. Tilt the container and carefully layer isopropyl alcohol on top of the liquid. DNA strands will become visible as white, stringy precipitates where the two layers meet.
There you have it – a step-by-step guide on how to extract DNA from a banana. This experiment provides a simple and fun way to learn about the basic principles of DNA extraction. Remember, DNA exists in all living organisms, and by extracting it from a banana, you gain a new appreciation for the building blocks of life. So go ahead, give it a try, and amaze your friends with your newfound knowledge!